Review



rabbit anti ccr2 polyclonal serum  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Novus Biologicals rabbit anti ccr2 polyclonal serum
    WT C57BL/6J mice were treated with anti-IFNAR1 mAb plus isotype control <t>or</t> <t>anti-CCR2</t> mAb (25 μg/mouse) and subcutaneously inoculated with 10 2 FFU of WNV 1 day later. Two additional doses of isotype control or anti-CCR2 mAb were administered at 1 and 3 dpi. At 5 dpi, mice were administered fluorescently conjugated dextrans by oral gavage. (A-B and E-F) Confocal microscopy images of duodenal sections at 5 dpi. (A) WNV antigen (red), monocytes (CCR2, green), EpCAM (white), and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel). (B) Quantification of CCR2-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field. (C and D) Serum levels of 10 (C) and 250 (D) kDa dextran. (E and F) Quantification of WNV antigen-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field (E) or as percentage of total EpCAM-positive epithelial cells per field (F). (G and H) Hematoxylin and eosin staining of liver sections from mice at 5 dpi. Scale bars, 100 μm (G). (H) Quantitation of liver injury. (I–K) Confocal microscopy images of cerebral cortex sections at 5 dpi showing Iba1 (white) and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel) (I). (J) Iba1-positive area was quantified from at least 15 microglia/macrophage cells per field and normalized to the number of microglia/macrophage nuclei counted. (K) Numbers of Iba1-positive microglia/macrophages per field. Each data point is derived from at least three independent fields per mouse brain (J–K). (L–O) Cytokine levels of TNF (L and M) and IL-6 (N and O) in serum (L and N) and brain homogenates (M and O). (P–S) WNV RNA levels in the serum (P), spleen (Q), liver (R), and brain (S) at 5 dpi. Bars indicate median values from two experiments; dotted lines show LODs; n = 10 mice (A–F and I–S); n = 6 mice (G–H). Statistical analysis: Mann-Whitney test (ns: non-significant, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).
    Rabbit Anti Ccr2 Polyclonal Serum, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ccr2+polyclonal+serum/CCR2+Antibody+-+BSA+Free/pmc12878153-267-27-32
    Average 94 stars, based on 15 article reviews
    rabbit anti ccr2 polyclonal serum - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "MyD88 signaling in myeloid cells induces gastrointestinal tract injury and systemic inflammation after West Nile virus infection"

    Article Title: MyD88 signaling in myeloid cells induces gastrointestinal tract injury and systemic inflammation after West Nile virus infection

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.116852

    WT C57BL/6J mice were treated with anti-IFNAR1 mAb plus isotype control or anti-CCR2 mAb (25 μg/mouse) and subcutaneously inoculated with 10 2 FFU of WNV 1 day later. Two additional doses of isotype control or anti-CCR2 mAb were administered at 1 and 3 dpi. At 5 dpi, mice were administered fluorescently conjugated dextrans by oral gavage. (A-B and E-F) Confocal microscopy images of duodenal sections at 5 dpi. (A) WNV antigen (red), monocytes (CCR2, green), EpCAM (white), and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel). (B) Quantification of CCR2-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field. (C and D) Serum levels of 10 (C) and 250 (D) kDa dextran. (E and F) Quantification of WNV antigen-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field (E) or as percentage of total EpCAM-positive epithelial cells per field (F). (G and H) Hematoxylin and eosin staining of liver sections from mice at 5 dpi. Scale bars, 100 μm (G). (H) Quantitation of liver injury. (I–K) Confocal microscopy images of cerebral cortex sections at 5 dpi showing Iba1 (white) and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel) (I). (J) Iba1-positive area was quantified from at least 15 microglia/macrophage cells per field and normalized to the number of microglia/macrophage nuclei counted. (K) Numbers of Iba1-positive microglia/macrophages per field. Each data point is derived from at least three independent fields per mouse brain (J–K). (L–O) Cytokine levels of TNF (L and M) and IL-6 (N and O) in serum (L and N) and brain homogenates (M and O). (P–S) WNV RNA levels in the serum (P), spleen (Q), liver (R), and brain (S) at 5 dpi. Bars indicate median values from two experiments; dotted lines show LODs; n = 10 mice (A–F and I–S); n = 6 mice (G–H). Statistical analysis: Mann-Whitney test (ns: non-significant, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).
    Figure Legend Snippet: WT C57BL/6J mice were treated with anti-IFNAR1 mAb plus isotype control or anti-CCR2 mAb (25 μg/mouse) and subcutaneously inoculated with 10 2 FFU of WNV 1 day later. Two additional doses of isotype control or anti-CCR2 mAb were administered at 1 and 3 dpi. At 5 dpi, mice were administered fluorescently conjugated dextrans by oral gavage. (A-B and E-F) Confocal microscopy images of duodenal sections at 5 dpi. (A) WNV antigen (red), monocytes (CCR2, green), EpCAM (white), and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel). (B) Quantification of CCR2-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field. (C and D) Serum levels of 10 (C) and 250 (D) kDa dextran. (E and F) Quantification of WNV antigen-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field (E) or as percentage of total EpCAM-positive epithelial cells per field (F). (G and H) Hematoxylin and eosin staining of liver sections from mice at 5 dpi. Scale bars, 100 μm (G). (H) Quantitation of liver injury. (I–K) Confocal microscopy images of cerebral cortex sections at 5 dpi showing Iba1 (white) and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel) (I). (J) Iba1-positive area was quantified from at least 15 microglia/macrophage cells per field and normalized to the number of microglia/macrophage nuclei counted. (K) Numbers of Iba1-positive microglia/macrophages per field. Each data point is derived from at least three independent fields per mouse brain (J–K). (L–O) Cytokine levels of TNF (L and M) and IL-6 (N and O) in serum (L and N) and brain homogenates (M and O). (P–S) WNV RNA levels in the serum (P), spleen (Q), liver (R), and brain (S) at 5 dpi. Bars indicate median values from two experiments; dotted lines show LODs; n = 10 mice (A–F and I–S); n = 6 mice (G–H). Statistical analysis: Mann-Whitney test (ns: non-significant, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).

    Techniques Used: Control, Confocal Microscopy, Staining, Quantitation Assay, Derivative Assay, MANN-WHITNEY

    Related Articles

    Incubation:

    Article Title: MyD88 signaling in myeloid cells induces gastrointestinal tract injury and systemic inflammation after West Nile virus infection.
    Article Snippet: Slides were blocked for 1 h at room temperature in Tris-buffered saline (TBS) solution containing 1% bovine serum albumin (BSA, Sigma), 5% normal donkey serum (Sigma), and 0.1% Triton X-100 (Sigma). .. Sections were incubated overnight at 4 ◦ C with rat anti-WNV hyperimmune serum 68 (1:750 dilution), rabbit anti-EpCAM polyclonal serum (1:2000, Abcam) and goat anti-Iba1 polyclonal serum (1:500, ThermoFisher) or rabbit anti-CCR2 polyclonal serum (1:500, Novus Biologicals) diluted in 1X TBS containing 1% BSA, 3% normal donkey serum, and 0.1% Triton X-100. .. After three rinses with PBS, the sections were incubated with secondary antibodies in the dark for 2 h at room temperature including AF594-conjugated donkey anti-rat antibody (1:1000, ThermoFisher), AF647-conjugated donkey anti-rabbit antibody (1:1000, ThermoFisher), and fluorescein-conjugated Ulex Europaeus Agglutinin I (UEA-1, 1:2000, ThermoFisher) or AF488-conjugated donkey anti-goat antibody (1:1000, ThermoFisher) diluted in TBS containing 1% BSA, 3% normal donkey serum, and 0.1% Triton X-100.

    Article Title: MyD88 signaling in myeloid cells induces gastrointestinal tract injury and systemic inflammation after West Nile virus infection
    Article Snippet: Slides were blocked for 1 h at room temperature in Tris-buffered saline (TBS) solution containing 1% bovine serum albumin (BSA, Sigma), 5% normal donkey serum (Sigma), and 0.1% Triton X-100 (Sigma). .. Sections were incubated overnight at 4°C with rat anti-WNV hyperimmune serum (1:750 dilution), rabbit anti-EpCAM polyclonal serum (1:2000, Abcam) and goat anti-Iba1 polyclonal serum (1:500, ThermoFisher) or rabbit anti-CCR2 polyclonal serum (1:500, Novus Biologicals) diluted in 1X TBS containing 1% BSA, 3% normal donkey serum, and 0.1% Triton X-100. .. After three rinses with PBS, the sections were incubated with secondary antibodies in the dark for 2 h at room temperature including AF594-conjugated donkey anti-rat antibody (1:1000, ThermoFisher), AF647-conjugated donkey anti-rabbit antibody (1:1000, ThermoFisher), and fluorescein-conjugated Ulex Europaeus Agglutinin I (UEA-1, 1:2000, ThermoFisher) or AF488-conjugated donkey anti-goat antibody (1:1000, ThermoFisher) diluted in TBS containing 1% BSA, 3% normal donkey serum, and 0.1% Triton X-100.



    Similar Products

    rabbit anti ccr2 polyclonal serum  (Novus Biologicals)
    94
    Novus Biologicals rabbit anti ccr2 polyclonal serum
    WT C57BL/6J mice were treated with anti-IFNAR1 mAb plus isotype control <t>or</t> <t>anti-CCR2</t> mAb (25 μg/mouse) and subcutaneously inoculated with 10 2 FFU of WNV 1 day later. Two additional doses of isotype control or anti-CCR2 mAb were administered at 1 and 3 dpi. At 5 dpi, mice were administered fluorescently conjugated dextrans by oral gavage. (A-B and E-F) Confocal microscopy images of duodenal sections at 5 dpi. (A) WNV antigen (red), monocytes (CCR2, green), EpCAM (white), and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel). (B) Quantification of CCR2-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field. (C and D) Serum levels of 10 (C) and 250 (D) kDa dextran. (E and F) Quantification of WNV antigen-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field (E) or as percentage of total EpCAM-positive epithelial cells per field (F). (G and H) Hematoxylin and eosin staining of liver sections from mice at 5 dpi. Scale bars, 100 μm (G). (H) Quantitation of liver injury. (I–K) Confocal microscopy images of cerebral cortex sections at 5 dpi showing Iba1 (white) and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel) (I). (J) Iba1-positive area was quantified from at least 15 microglia/macrophage cells per field and normalized to the number of microglia/macrophage nuclei counted. (K) Numbers of Iba1-positive microglia/macrophages per field. Each data point is derived from at least three independent fields per mouse brain (J–K). (L–O) Cytokine levels of TNF (L and M) and IL-6 (N and O) in serum (L and N) and brain homogenates (M and O). (P–S) WNV RNA levels in the serum (P), spleen (Q), liver (R), and brain (S) at 5 dpi. Bars indicate median values from two experiments; dotted lines show LODs; n = 10 mice (A–F and I–S); n = 6 mice (G–H). Statistical analysis: Mann-Whitney test (ns: non-significant, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).
    Rabbit Anti Ccr2 Polyclonal Serum, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+ccr2+polyclonal+serum/CCR2+Antibody+-+BSA+Free/pmc12878153-267-27-32
    Average 94 stars, based on 1 article reviews
    rabbit anti ccr2 polyclonal serum - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    WT C57BL/6J mice were treated with anti-IFNAR1 mAb plus isotype control or anti-CCR2 mAb (25 μg/mouse) and subcutaneously inoculated with 10 2 FFU of WNV 1 day later. Two additional doses of isotype control or anti-CCR2 mAb were administered at 1 and 3 dpi. At 5 dpi, mice were administered fluorescently conjugated dextrans by oral gavage. (A-B and E-F) Confocal microscopy images of duodenal sections at 5 dpi. (A) WNV antigen (red), monocytes (CCR2, green), EpCAM (white), and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel). (B) Quantification of CCR2-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field. (C and D) Serum levels of 10 (C) and 250 (D) kDa dextran. (E and F) Quantification of WNV antigen-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field (E) or as percentage of total EpCAM-positive epithelial cells per field (F). (G and H) Hematoxylin and eosin staining of liver sections from mice at 5 dpi. Scale bars, 100 μm (G). (H) Quantitation of liver injury. (I–K) Confocal microscopy images of cerebral cortex sections at 5 dpi showing Iba1 (white) and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel) (I). (J) Iba1-positive area was quantified from at least 15 microglia/macrophage cells per field and normalized to the number of microglia/macrophage nuclei counted. (K) Numbers of Iba1-positive microglia/macrophages per field. Each data point is derived from at least three independent fields per mouse brain (J–K). (L–O) Cytokine levels of TNF (L and M) and IL-6 (N and O) in serum (L and N) and brain homogenates (M and O). (P–S) WNV RNA levels in the serum (P), spleen (Q), liver (R), and brain (S) at 5 dpi. Bars indicate median values from two experiments; dotted lines show LODs; n = 10 mice (A–F and I–S); n = 6 mice (G–H). Statistical analysis: Mann-Whitney test (ns: non-significant, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).

    Journal: Cell reports

    Article Title: MyD88 signaling in myeloid cells induces gastrointestinal tract injury and systemic inflammation after West Nile virus infection

    doi: 10.1016/j.celrep.2025.116852

    Figure Lengend Snippet: WT C57BL/6J mice were treated with anti-IFNAR1 mAb plus isotype control or anti-CCR2 mAb (25 μg/mouse) and subcutaneously inoculated with 10 2 FFU of WNV 1 day later. Two additional doses of isotype control or anti-CCR2 mAb were administered at 1 and 3 dpi. At 5 dpi, mice were administered fluorescently conjugated dextrans by oral gavage. (A-B and E-F) Confocal microscopy images of duodenal sections at 5 dpi. (A) WNV antigen (red), monocytes (CCR2, green), EpCAM (white), and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel). (B) Quantification of CCR2-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field. (C and D) Serum levels of 10 (C) and 250 (D) kDa dextran. (E and F) Quantification of WNV antigen-positive cells in the GI tract, expressed as percentage of total Hoechst 33258-positive cells per field (E) or as percentage of total EpCAM-positive epithelial cells per field (F). (G and H) Hematoxylin and eosin staining of liver sections from mice at 5 dpi. Scale bars, 100 μm (G). (H) Quantitation of liver injury. (I–K) Confocal microscopy images of cerebral cortex sections at 5 dpi showing Iba1 (white) and nuclei (Hoechst 33258, blue). Scale bars, 100 μm (upper panel) or 50 μm (lower panel) (I). (J) Iba1-positive area was quantified from at least 15 microglia/macrophage cells per field and normalized to the number of microglia/macrophage nuclei counted. (K) Numbers of Iba1-positive microglia/macrophages per field. Each data point is derived from at least three independent fields per mouse brain (J–K). (L–O) Cytokine levels of TNF (L and M) and IL-6 (N and O) in serum (L and N) and brain homogenates (M and O). (P–S) WNV RNA levels in the serum (P), spleen (Q), liver (R), and brain (S) at 5 dpi. Bars indicate median values from two experiments; dotted lines show LODs; n = 10 mice (A–F and I–S); n = 6 mice (G–H). Statistical analysis: Mann-Whitney test (ns: non-significant, ** p < 0.01, *** p < 0.001, and **** p < 0.0001).

    Article Snippet: Sections were incubated overnight at 4°C with rat anti-WNV hyperimmune serum (1:750 dilution), rabbit anti-EpCAM polyclonal serum (1:2000, Abcam) and goat anti-Iba1 polyclonal serum (1:500, ThermoFisher) or rabbit anti-CCR2 polyclonal serum (1:500, Novus Biologicals) diluted in 1X TBS containing 1% BSA, 3% normal donkey serum, and 0.1% Triton X-100.

    Techniques: Control, Confocal Microscopy, Staining, Quantitation Assay, Derivative Assay, MANN-WHITNEY